Elabscience
Total Sulfhydryl Group/Total Thiol (-SH) Colorimetric Assay Kit
- SKU:
- E-BC-K265-M
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
Detection principle
Sulfhydryl compounds react with 5,5' -dithiobis (2-nitrobenzoic acid) under neutral or alkaline conditions to produce a yellow product which have a maximum absorption peak at 412 nm. Measure the OD value and calculate the total mercapto content indirectly.
Performance characteristics
| Synonyms | T-SH |
| Sample type | Serum, plasma, animal tissue |
| Sensitivity | 9.91 μmol/L |
| Detection range | 9.91-1000 μmol/L |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 20 min |
| Precision | Average inter-assay CV: 2.9%Average intra-assay CV: 2.5% |
| Other instruments required | Micropipettor, Water bath, Incubator, Vortex mixer, Centrifuge |
| Other reagents required | Absolute ethyl alcohol |
| Storage | 2-8℃ |
| Valid period | 6 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (9.91-1000 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type |
Dilution factor |
|
Human plasma |
4-6 |
|
Human serum |
4-6 |
|
Human urine |
1 |
|
10% Rat liver tissue homogenate |
4-6 |
|
Rabbit serum |
3-5 |
|
Porcine serum |
4-6 |
|
10% Rat kidney tissue homogenate |
4-6 |
|
10% Rat spleen tissue homogenate |
4-6 |
Note: The diluent is normal saline (0.9% NaCl).
Additional Information
Detection instrument: |
Microplate reader(410 nm-420 nm,optimum wavelength: 412 nm) |
Detection method: |
Colorimetric method |
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