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Elabscience

Malondialdehyde (MDA) Colorimetric Assay Kit (Cell Samples)

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SKU:
E-BC-K028-M
Weight:
0 KGS
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€364.00

Description

Detection principle

MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.

Performance characteristics

Synonyms MDA
Sample type Cells
Sensitivity 0.29 nmol/mL
Detection range 0.29-100 nmol/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 65 min
Precision Average inter-assay CV: 3.5%Average intra-assay CV: 3.3%
Other instruments required Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer
Other reagents required Absolute ethanol
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.29-100 nmol/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

A549 cells

1

HepG2 cells

1

293T cells

1

Note: The diluent is reagent 5 working solution.

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Additional Information

Detection instrument:
Microplate reader(530-540 nm,optimum wavelength: 532 nm)
Detection method:
Colorimetric method
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