Elabscience
Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)
- SKU:
- E-BC-K025-M
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
Detection principle
MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.
Performance characteristics
| Synonyms | MDA |
| Sample type | Serum,plasma,animal tissue |
| Sensitivity | 1.13 μmol/L |
| Detection range | 2.92-40 μmol/L |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 65 min |
| Precision | Average inter-assay CV: 7.2%Average intra-assay CV: 4.1% |
| Other instruments required | Micropipettor, Vortex mixer, Incubator, Centrifuge, Magnetic Stirrers |
| Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol |
| Storage | 2-8℃ |
| Valid period | 6 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the formal experiment and the detection range (2.92-40 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type |
Dilution factor |
|
Human serum |
1 |
|
Human plasma |
1 |
|
Rat serum |
1 |
|
Rat plasma |
1 |
|
Mouse serum |
1 |
|
Mouse plasma |
1 |
|
10% Rat heart tissue homogenate |
1 |
|
10% Rat liver tissue homogenate |
1 |
|
10% Rat spleen tissue homogenate |
1 |
|
10% Rat lung tissue homogenate |
1 |
|
10% Rat kidney tissue homogenate |
1 |
|
10% Rat brain tissue homogenate |
1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Additional Information
Detection instrument: |
Microplate reader(530-540 nm,optimum wavelength: 532 nm) |
Detection method: |
Colorimetric method |
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