Elabscience

Myeloperoxidase (MPO) Peroxidation Activity Fluorometric Assay Kit

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E-BC-F013
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  • Myeloperoxidase (MPO) Peroxidation Activity Fluorometric Assay Kit
  • Myeloperoxidase (MPO) Peroxidation Activity Fluorometric Assay Kit
  • Myeloperoxidase (MPO) Peroxidation Activity Fluorometric Assay Kit
€412.00

Description

Detection principle

Under the catalysis of peroxidase, hydrogen peroxide can oxidize the non-fluorescent probe into the fluorescent substance, and its fluorescence intensity is proportional to the total peroxidase activity in the sample. This kit specifically inhibits the peroxidase activity of MPO in the sample through an MPO enzyme inhibitor, thus distinguishing the peroxidase activity of MPO in the sample from that of other peroxidases.

Performance characteristics

Synonyms MPO
Sample type Serum, plasma, animal tissue
Sensitivity 0.001 U/L
Detection range 0.001 - 1.26 U/L
Detection method Fluorescence method
Assay type Enzyme Activity
Assay time 20 min
Precision Average inter-assay CV: 5.4%Average intra-assay CV: 1%
Other instruments required Micropipette, Vortex mixer, Water bath
Storage -20℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.001 - 1.26 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Porcine serum

5-10

Rabbit serum

3-5

Rat serum

2-5

Mouse serum

10-20

Mouse plasma

30-50

Horse serum

2-5

10% Rat heart tissue homogenate

1

10% Rat lung tissue homogenate

1

Note: The diluent is reagent 1.

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Additional Information

Detection method:
Fluorescence method
Detection instrument:
Fluorescence microplate reader (Ex/Em=535 nm/587 nm)
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