Thiobarbituric Acid Reactants (TBARS) Fluorometric Assay Kit

Detection principle

TBARS and TBA can react under high temperature and acid conditions and then form a pink compound, the concentration of which is linearly related to the concentration of TBARS in the sample. The TBARS concentration can be calculated by measuring the fluorescence values at the excitation wavelength of 530 nm and the emission wavelength of 550 nm.

Performance characteristics

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.09-10 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).