ProSci

IFN-gamma Antibody

(No reviews yet) Write a Review
SKU:
XP-5155
Weight:
0 KGS
Shipping:
Calculated at Checkout
  • IFN-gamma Antibody
  • IFN-gamma Antibody
  • IFN-gamma Antibody
  • IFN-gamma Antibody
  • IFN-gamma Antibody
  • IFN-gamma Antibody
  • IFN-gamma Antibody
  • IFN-gamma Antibody
  • IFN-gamma Antibody
  • IFN-gamma Antibody
€393.42

Description

IFN-gamma Antibody (biotin) | XP-5155Bt | ProSci

 

Host: Rabbit

 

Reactivity: Human

 

Homology: N/A

 

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIFN-gamma (human Interferon-gamma) .

 

Research Area: Immunology, Chemokines & Cytokines, Antibody Pairs

 

Tested Application: E, WB

 

Application: ELISA:
Sandwich:
To detect hIFN-γ by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IFN-γ (XP-5155) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIFN-γ.
Western Blot:
To detect hIFN-γ by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIFN-γ is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

 

Specificiy: N/A

 

Positive Control 1: N/A

 

Positive Control 2: N/A

 

Positive Control 3: N/A

 

Positive Control 4: N/A

 

Positive Control 5: N/A

 

Positive Control 6: N/A

 

Molecular Weight: N/A

 

Validation: N/A

 

Isoform: N/A

 

Purification: Anti-hIFN-gamma specific antibody was purified by affinity chromatography and then biotinylated.

 

Clonality: Polyclonal

 

Clone: N/A

 

Isotype: N/A

 

Conjugate: Biotin

 

Physical State: Lyophilized

 

Buffer: N/A

 

Concentration: N/A

 

Storage Condition: IFN-gamma antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

 

Alternate Name: IFG, IFIInterferon gamma, Immune interferon, IFN-gamma

 

User Note: Centrifuge vial prior to opening.

 

BACKGROUND: IFN gamma receptor beta is part of the receptor for interferon gamma. This class II cytokine receptor pairs with CDw119 to form the IFN gamma receptor and is an integral part of the IFN gamma signal transduction pathway. CDw119 serves as the IFN gamma binding chain and associates with the IFN gamma beta chain which is required for receptor signaling. The extracellular portion of both the IFN gamma receptor alpha and beta chains must be species matched. The IFN gamma receptor beta chain is expressed on T and B cells, NK cells, monocytes/ macrophages, and fibroblasts. Binding of IFN gamma induces receptor dimerization, internalization, Jak1 and Jak2 protein kinase activation and, ultimately, STAT1 activation. It is also likely to interact with GAF. IFN gamma initiates and regulates a variety of immune responses and is required for signal transduction. Contains 2 fibronectin type III domains. Defects in IFN gamma Receptor beta are a cause of mendelian susceptibility to mycobacterial disease (MSMD) , a rare condition that confers predisposition to illness caused by several mycobacteria strains

View AllClose

Additional Information

Clonality:
Polyclonal
Tested Applications:
E, Neut, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Size:
0.1 mg
View AllClose