Human IFN-γ(Interferon Gamma) ELISA Kit Basic Information 

Feiyue's Human IFN-γ is an ELISA reagent for detection of IFN-γ antibody, plasma or cell with sensitivity, specificity and consistency.

Human IFN-γ(Interferon Gamma) Introduction

IFN-γ is a dimerized soluble cytokine that is the only member of the type II class of interferons. The existence of this interferon, which early in its history was known as immune interferon, was described by E. F. Wheelock as a product of human leukocytes stimulated with phytohemagglutinin, and by others as a product of antigen-stimulated lymphocytes. It was also shown to be produced in human lymphocytes. or tuberculin-sensitized mouse peritoneal lymphocytes challenged with Mantoux test (PPD); the resulting supernatants were shown to inhibit growth of vesicular stomatitis virus. Those reports also contained the basic observation underlying the now widely employed interferon gamma release assay used to test for tuberculosis. In humans, the IFNγ protein is encoded by the IFNG gene.

Human IFN-γ(Interferon Gamma) ELISA Kit Test Method

This kit uses sandwich ELISA to detect the concentration of Human IFN-γ.

Human IFN-γ -specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti- Human IFN-γ antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming opticalsignal measured by microplate reader.The concentration of Human IFN-γ in the sample can be calculated by comparing the absorbance of the sample with the standard curve.  

Reference:

1. GRCh38: Ensembl release 89: ENSG00000111537 - Ensembl, May 2017.
2. GRCm38: Ensembl release 89: ENSMUSG00000055170 - Ensembl, May 2017.
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.