sIgA(Secretory Immunoglobulin A) Introduction

Immunoglobulin A (IgA, also referred to as sIgA in its secretory form) is an antibody that plays a crucial role in the immune function of mucous membranes. The amount of IgA produced in association with mucosal membranes is greater than all other types of antibody combined. In absolute terms, between three and five grams are secreted into the intestinal lumen each day. This represents up to 15% of total immunoglobulins produced throughout the body. In the blood, IgA interacts with an Fc receptor called FcαRI (or CD89), which is expressed on immune effector cells, to initiate inflammatory reactions. Ligation of FcαRI by IgA containing immune complexes causes antibody-dependent cell-mediated cytotoxicity (ADCC), degranulation of eosinophils and basophils, phagocytosis by monocytes, macrophages, and neutrophils, and triggering of respiratory burst activity by polymorphonuclear leukocytes

Mouse sIgA(Secretory Immunoglobulin A) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentMouseion of  sIgA(Secretory Immunoglobulin A) The microtiter plate provided in this kit has been pre-coated with an antibody specific to sIgA(Secretory Immunoglobulin A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to sIgA(Secretory Immunoglobulin A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substMousee solution is added, only those wells that contain of sIgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substMousee reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentMouseion of sIgA(Secretory Immunoglobulin A) in the samples is then determined by comparing the OD of the samples to the standard curve.

Reference:

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2.Holmgren J, Czerkinsky C (April 2005). "Mucosal immunity and vaccines". Nature Medicine. 11 (4 Suppl): S45–53. doi:10.1038/nm1213. PMID 15812489.

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