Elabscience
Malondialdehyde (MDA) Colorimetric Assay Kit (Cell Samples)
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- SKU:
- E-BC-K028-M
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
Detection principle
MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.
Performance characteristics
| Synonyms | MDA |
| Sample type | Cells |
| Sensitivity | 0.29 nmol/mL |
| Detection range | 0.29-100 nmol/mL |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 65 min |
| Precision | Average inter-assay CV: 3.5%Average intra-assay CV: 3.3% |
| Other instruments required | Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer |
| Other reagents required | Absolute ethanol |
| Storage | 2-8℃ |
| Valid period | 6 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.29-100 nmol/mL).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type |
Dilution factor |
|
A549 cells |
1 |
|
HepG2 cells |
1 |
|
293T cells |
1 |
Note: The diluent is reagent 5 working solution.
Additional Information
Detection instrument: |
Microplate reader(530-540 nm,optimum wavelength: 532 nm) |
Detection method: |
Colorimetric method |
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