Elabscience

Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)

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E-BC-K025-M
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  • Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)
  • Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)
  • Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)
€295.00 - €386.00

Description

Detection principle

MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.

Performance characteristics

Synonyms MDA
Sample type Serum,plasma,animal tissue
Sensitivity 1.13 μmol/L
Detection range 2.92-40 μmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 65 min
Precision Average inter-assay CV: 7.2%Average intra-assay CV: 4.1%
Other instruments required Micropipettor, Vortex mixer, Incubator, Centrifuge, Magnetic Stirrers
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the formal experiment and the detection range (2.92-40 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Human plasma

1

Rat serum

1

Rat plasma

1

Mouse serum

1

Mouse plasma

1

10% Rat heart tissue homogenate

1

10% Rat liver tissue homogenate

1

10% Rat spleen tissue homogenate

1

10% Rat lung tissue homogenate

1

10% Rat kidney tissue homogenate

1

10% Rat brain tissue homogenate

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

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Additional Information

Detection instrument:
Microplate reader(530-540 nm,optimum wavelength: 532 nm)
Detection method:
Colorimetric method
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