Elabscience
Peroxidase (POD) Activity Assay Kit (Plant samples)
- SKU:
- E-BC-K227-M
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
Detection principle
Plant peroxidase, a member of the superfamily of peroxidase, catalyzes the redox reaction between H2O2 and various reductants. The plant peroxidase has the same general structure and consists of iron porphyrin IX and ten α-helixes. Based on the difference of primary structure, the superfamily of plant peroxidase can be divided into three types: class I (intracellular type), class II (extracellular type of fungi) and class III (secreted type of plant).
Performance characteristics
| Synonyms | POD |
| Sample type | Plant tissue |
| Sensitivity | 0.01 U/mL |
| Detection range | 0.01–100 U/mL |
| Detection method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 60 min |
| Precision | Average inter-assay CV: 5%Average intra-assay CV: 3.2% |
| Other instruments required | Spectrophotometer (240 nm), Micropipettor, Vortex mixer, Centrifuge, 37℃ Incubator |
| Other reagents required | PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 6 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-100 U/mL).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type |
Dilution factor |
|
10% Green pepper tissue homogenate |
1 |
|
10% Chive leaf tissue homogenate |
1 |
|
10% Photinia leaf tissue homogenate |
1 |
|
10% Epipremnum aureum tissue homogenate |
1 |
|
10% Mushrooms tissue homogenate |
1 |
|
10% White radish tissue homogenate |
1 |
Note: The diluent is PBS (0.01 M, pH 7.4).
Additional Information
Detection method: |
Colorimetric method |
Detection instrument: |
Microplate reader(410 nm-430 nm,optimum wavelength: 420 nm) |
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