Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit

Detection principle

A variety of antioxidant macromolecules, antioxidant molecules and enzymes in a system can eliminate all kinds of reactive oxygen species and prevent oxidative stress induced by reactive oxygen species. The total level reflect the total antioxidant capacity in the system. Many antioxidants in the body can reduce Fe3+ to Fe2+ and Fe2+ can form stable complexes with phenanthroline substance. The antioxidant capacity (T-AOC) can be calculated by measuring the absorbance at 520 nm.

Performance characteristics

Images

Significant decrease of T-AOC was observed in AFB1 group (**P<0.01 vs. Control). Significant increase of T-AOC was observed in B10 treated mice (##P<0.01 vs. AFB1 group).

X Li et al investigate that Bacillus amyloliquefaciens B10 alleviate liver injury induced by aflatoxin B1 as feed additive. Total antioxidant capacity (T-AOC) in mouse liver tissue was determined using T-AOC colorimetric assay kit (E-BC-K136-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.62-190.43 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).