Elabscience
Vitamin C (VC) Colorimetric Assay Kit
- SKU:
- E-BC-K034-M
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
Detection principle
The most obvious chemical activity of VC is that reduce Fe3+ to Fe2+, then promote iron absorption in the intestine, promote the storage and utilization of iron. Fe3+ react immediately with reducing ascorbic acid to form Fe2+. then Fe2+ react with phenanthroline and the color developing reaction occurs. The content of vitamin C in sample can be determined. Measure the OD value and calculate the VC content indirectly.
Performance characteristics
| Synonyms | VC,Ascorbic acid |
| Sample type | Serum,plasma,tissue |
| Sensitivity | 0.31 μg/mL |
| Detection range | 0.31-20 μg/mL |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 50min |
| Precision | Average inter-assay CV: 6.1%Average intra-assay CV: 2.2% |
| Other instruments required | Micropipettor, Centrifuge, Incubator, Vortex mixer |
| Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), Absolute ethanol |
| Storage | 2-8℃ |
| Valid period | 6 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.31-20 μg/mL).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type |
Dilution factor |
|
Human serum |
1 |
|
Mouse serum |
1 |
|
Chicken serum |
1 |
|
Horse serum |
1 |
|
10% Mouse kidney tissue homogenate |
1 |
|
10% Mouse liver tissue homogenate |
1 |
|
10% Mouse lung tissue homogenate |
1 |
|
10% Mouse spleen tissue homogenate |
1 |
|
10% Rat liver tissue homogenate |
1 |
|
10% Rat heart tissue homogenate |
1 |
|
10% Plant tissue homogenate |
1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Additional Information
Detection instrument: |
Microplate reader(530-540 nm,optimum wavelength: 536 nm) |
Detection method: |
Colorimetric method |
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