Human ACE2(Angiotensin-converting enzyme 2) ELISA Kit Basic Information 

Feiyue's Human ACE2(Angiotensin-converting enzyme 2) ELISA Kit is an ELISA reagent for detection of ACE2(Angiotensin-converting enzyme 2), plasma or cell with sensitivity, specificity and consistency.

ACE2(Angiotensin-converting enzyme 2) Introduction 

Angiotensin-converting enzyme 2 (ACE2) is an enzyme that can be found either attached to the membrane of cells (mACE2) in the intestines, kidney, testis, gallbladder, and heart or in a soluble form (sACE2).Both membrane bound and soluble ACE2 are integral parts of the renin–angiotensin–aldosterone system (RAAS) that exists to keep the body's blood pressure in check. While mACE2 does not appear to factor into the harmful phase of RAAS (the increase of blood pressure), its existence is vital in order for the enzyme ADAM17 to cleave its extracellular domain to create soluble ACE2 (sACE2). Soluble ACE2 lowers blood pressure by catalyzing the hydrolysis of angiotensin II (a vasoconstrictor peptide) into angiotensin (1–7) (a vasodilator) which in turns binds to MasR receptors creating localized vasodilation and hence decreasing blood pressure.This decrease in blood pressure makes the entire process a promising drug target for treating cardiovascular diseases.mACE2 also serves as the entry point into cells for some coronaviruses, including HCoV-NL63, SARS-CoV, and SARS-CoV-2.

Human ACE2(Angiotensin-converting enzyme 2) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of ACE2(Angiotensin-converting enzyme 2).ACE2(Angiotensin-converting enzyme 2)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-ACE2(Angiotensin-converting enzyme 2) antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming optical signal measured by microplate reader.The concentration of ACE2(Angiotensin-converting enzyme 2) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.  

Reference:

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