CRP(C-Reactive Protein) Introduction

C-reactive protein (CRP) is an annular (ring-shaped) pentameric protein found in blood plasma, whose circulating concentrations rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. Its physiological role is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via C1q.

Human CRP(C-Reactive Protein) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concent Humanion of  CRP(C-Reactive Protein).CRP(C-Reactive Protein)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilizedantibody. A sandwich complex is formed by additional  CRP(C-Reactive Protein) antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming optical signal measured by microplate reader.The concentHumanion of  CRP(C-Reactive Protein) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.  

References

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