Human GAL3(Galectin-3) Introduction

GAL3 is a protein that in humans is encoded by the LGALS3 gene. Galectin-3 is a member of the lectin family, of which 14 mammalian galectins have been identified. GAL3 is approximately 30 kDa and, like all galectins,like all galectins, contains a carbohydrate-recognition-binding domain (CRD) of about 130 amino acids that enable the specific binding of β-galactosides. Galectin-3 (Gal-3) is also a member of the beta-galactoside-binding protein family that plays an important role in cell-cell adhesion, cell-matrix interactions, macrophage activation, angiogenesis, metastasis, apoptosis. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21–q22. Galectin-3 is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space.

Human GAL3(Galectin-3) ELISA Kit Test Method

This kit uses sandwich ELISA to detect the concentration of Human GAL3.

Human GAL3-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human GAL3 antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming opticalsignal measured by microplate reader.The concentration of Human GAL3 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.  

Reference:

1. GRCh38: Ensembl release 89: ENSG00000131981 - Ensembl, May 2017.
2. GRCm38: Ensembl release 89: ENSMUSG00000050335 - Ensembl, May 2017.
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.