Human IgG(Immunoglobulin G) Introduction

LgG is a type of antibody. Representing approximately 75% of serum antibodies in humans, IgG is the most common type of antibody found in blood circulation. IgG molecules are created and released by plasma B cells. Each IgG antibody has two paratopes. Antibodies are major components of humoral immunity. IgG is the main type of antibody found in blood and extracellular fluid, allowing it to control infection of body tissues. By binding many kinds of pathogens such as viruses, bacteria, and fungi, IgG protects the body from infection. IgG antibodies are generated following class switching and maturation of the antibody response, thus they participate predominantly in the secondary immune response.

Human IgG(Immunoglobulin G) ELISA Kit Test Method

This kit uses sandwich ELISA to detect the concentration of Human IgG.

Human IgG-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human IgG antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentRabbition of Human IgG in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1. Vidarsson, Gestur; Dekkers, Gillian; Rispens, Theo (2014). "IgG subclasses and allotypes: from structure to effector functions". Frontiers in Immunology. 5: 520. doi:10.3389/fimmu.2014.00520. ISSN 1664-3224. PMC 4202688. PMID 25368619.
2. Mallery DL, McEwan WA, Bidgood SR, Towers GJ, Johnson CM, James LC (2010). "Antibodies mediate intracellular immunity through tripartite motif-containing 21 (TRIM21)". Proceedings of the National Academy of Sciences, USA. 107 (46): 19985–19990. Bibcode:2010PNAS..10719985M. doi:10.1073/pnas.1014074107. PMC 2993423. PMID 21045130.
3. Vidarsson, Gestur; Dekkers, Gillian; Rispens, Theo (2014). "IgG subclasses and allotypes: from structure to effector functions". Frontiers in Immunology. 5: 520. doi:10.3389/fimmu.2014.00520. ISSN 1664-3224. PMC 4202688. PMID 25368619.