Human IL-8(Interleukin 8) ELISA Kit Basic Information 

Feiyue’s Human IL-8 is an ELISA reagent for detection of IL-8 in human serum, plasma or cell with sensitivity, specificity and consistency.

Human IL-8(Interleukin 8) Introduciton

Interleukin 8 (IL-8 or chemokine (CXC motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. Endothelial cells store IL-8 in their storage vesicles, the Weibel-Palade bodies. In humans, the interleukin-8 protein is encoded by the CXCL8 gene. IL-8 is initially produced as a precursor peptide of 99 amino acids which then undergoes cleavage to create several active IL-8 isoforms. In culture, a 72 amino acid peptide is the major form secreted by macrophages.

Human IL-8(Interleukin 8) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of Human IL-8.

Human IL-8-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human IL-8 antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human IL-8 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1.GRCh38: Ensembl release 89: ENSG00000169429-Ensembl, May 2017

2."Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

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4.Wolff B, Burns AR, Middleton J, Rot A (1998). "Endothelial cell "memory" of inflammatory stimulation: human venular endothelial cells store interleukin 8 in Weibel-Palade bodies". J. Exp. Med. 188 (9): 1757–62. doi:10.1084/jem.188.9.1757. PMC 2212526. PMID 9802987.