Feiyuebio

Human MMP-2(Matrix Metalloproteinase 2) ELISA Kit

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SKU:
FY-EH4085
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0 KGS
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  • Human MMP-2(Matrix Metalloproteinase 2) ELISA Kit
  • Human MMP-2(Matrix Metalloproteinase 2) ELISA Kit
€535.50

Description

Human MMP-2(Matrix Metalloproteinase 2) Introduciton

72 kDa type IV collagenase also known as matrix metalloproteinase-2 (MMP-2) and gelatinase A is an enzyme that in humans is encoded by the MMP2 gene.[5] The MMP2 gene is located on chromosome 16 at position 12.2. Activation of MMP-2 requires proteolytic processing. A complex of membrane type 1 MMP (MT1-MMP/MMP14) and tissue inhibitor of metalloproteinase 2 recruits pro-MMP 2 from the extracellular milieu to the cell surface. Activation then requires an active molecule of MT1-MMP and auto catalytic cleavage. Clustering of integrin chains promotes activation of MMP-2. Another factor that will support the activation of MMP-2 is cell-cell clustering. A wild-type activated leukocyte cell adhesion molecule (ALCAM) is also required to activate MMP-2.

Human MMP-2(Matrix Metalloproteinase 2) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of Human MMP-2.

Human MMP-2-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human MMP-2 antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human MMP-2 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

 

Reference:

  1. GRCh38: Ensembl release 89: ENSG00000087245 - Ensembl, May 2017
  2. GRCm38: Ensembl release 89: ENSMUSG00000031740 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. Devarajan P, Johnston JJ, Ginsberg SS, Van Wart HE, Berliner N (December 1992). "Structure and expression of neutrophil gelatinase cDNA. Identity with type IV collagenase from HT1080 cells". J. Biol. Chem. 267 (35): 25228–32. doi:10.1016/S0021-9258(19)74029-0. PMID 1460022.
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Additional Information

Size:
96T
Alias:
72 kDa type IV collagenase; 72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2; TBE-1; PEX; MMP2; CLG4A
Application:
MMP-2 ELISA Kit allows for the in vitro quantitative determination of MMP-2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Storage:
2-8 ℃ for 6 months
Sensitivity:
<0.191ng/ml
Species:
Human
UniProt ID:
P08253
CV %():
Intra-Assay: CV<8% Inter-Assay: CV<10%
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