Human OxLDL(Oxidized low-density lipoprotein) Introduciton

Oxidized LDL is a general term for LDL particles with oxidatively modified structural components. As a result from free radical attack, both lipid and protein parts of LDL can be oxidized in the vascular wall. Besides the oxidative reactions taking place in vascular wall, oxidized lipids in LDL can also be derived from oxidized dietary lipids. Oxidized LDL is known to associate with the development of atherosclerosis, and it is therefore widely studied as a potential risk factor of cardiovascular diseases. Atherogenicity of oxidized LDL has been explained by lack of recognition of oxidation-modified LDL structures by the LDL receptors, preventing the normal metabolism of LDL particles and leading eventually to development of atherosclerotic plaques. 

Human OxLDL(Oxidized low-density lipoprotein) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of Human OxLDL.

Human OxLDL-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human OxLDL antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human OxLDL in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1. "LDL and HDL: Bad and Good Cholesterol". Centers for Disease Control and Prevention. CDC. Retrieved 11 September 2017.
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3. Dashty M, Motazacker MM, Levels J, de Vries M, Mahmoudi M, Peppelenbosch MP, Rezaee F (2014). "Proteome of human plasma very low-density lipoprotein and low-density lipoprotein exhibits a link with coagulation and lipid metabolism". Thromb. Haemost. 111 (3): 518–530. doi:10.1160/TH13-02-0178. PMID 24500811.
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