NA/NE(Noradrenaline/Norepinephrine) ELISA Kit Basic Information 

Feiyue’s NA/NE is an ELISA reagent for detection of NA/NE in human serum, plasma or cell with sensitivity, specificity and consistency.

NA/NE(Noradrenaline/Norepinephrine) Introduciton

Norepinephrine (NE), also called noradrenaline (NA) or noradrenalin, is an organic chemical in the catecholamine family that functions in the brain and body as both a hormone and neurotransmitter. The general function of norepinephrine is to mobilize the brain and body for action. Norepinephrine release is lowest during sleep, rises during wakefulness, and reaches much higher levels during situations of stress or danger, in the so-called fight-or-flight response. In the brain, norepinephrine increases arousal and alertness, promotes vigilance, enhances formation and retrieval of memory, and focuses attention; it also increases restlessness and anxiety. In the rest of the body, norepinephrine increases heart rate and blood pressure, triggers the release of glucose from energy stores, increases blood flow to skeletal muscle, reduces blood flow to the gastrointestinal system, and inhibits voiding of the bladder and gastrointestinal motility.

NA/NE(Noradrenaline/Norepinephrine) ELISA Kit Test method

This kit was based on Competitive-ELISA detection method.

The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme -substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.

Reference:

1. Andersen, A. M. (1975). "Structural Studies of Metabolic Products of Dopamine. IV. Crystal and Molecular Structure of (−)-Noradrenaline". Acta Chem. Scand. 29b (8): 871–876. doi:10.3891/acta.chem.scand.29b-0871. PMID 1202890.
2. Aronson JK (February 2000). "'Where name and image meet'—the argument for 'adrenaline'". British Medical Journal. 320 (7233): 506–9. doi:10.1136/bmj.320.7233.506. PMC 1127537. PMID 10678871.
3. "(-)-noradrenaline". IUPHAR database. International Union of Basic and Clinical Pharmacology. Retrieved 2 January 2016.
4. "Norepinephrine". PubChem. Retrieved 6 November 2015.