Elabscience

Hydroxyl Free Radical Scavenging Capacity Assay Kit

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E-BC-K527-M
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  • Hydroxyl Free Radical Scavenging Capacity Assay Kit
  • Hydroxyl Free Radical Scavenging Capacity Assay Kit
  • Hydroxyl Free Radical Scavenging Capacity Assay Kit
€334.00 - €438.00

Description

Detection principle

Hydroxyl radical is a kind of reactive oxygen species, which can kill red blood cells, degrade DNA, cell membrane and polysaccharide compounds, causing damage to cell structure and function, and then leading to metabolic disorders in the body to cause diseases. The scavenging ability of hydroxyl free radical is one of the important indexes of the antioxidant ability of samples. It has been widely used in the research of antioxidant health care products and drugs.

Performance characteristics

Sample type Serum, plasma, animal tissue
Detection method Colorimetric method
Assay type Quantitative
Assay time 40 min
Precision Average inter-assay CV: 4.6%Average intra-assay CV: 4.1%
Other instruments required Micropipettor, Vortex mixer, Centrifuge
Other reagents required Absolute ethanol
Storage 2-8℃
Valid period 3 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the scavenging capacity detection range (10-50%). The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Dog serum

1

Rat serum

1

Cynomolgus monkey serum

1

10% Rat spleen tissue homogenate

1

10% Mouse liver tissue homogenate

1

10% Rat kidney tissue homogenate

1

10% Rat lung tissue homogenate

1

Note: The diluent is double distilled water.

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Additional Information

Detection method:
Colorimetric method
Detection instrument:
Microplate reader(500 nm-530 nm,optimum wavelength: 510 nm)
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