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Elabscience

Inhibition And Production Of Superoxide Anionic Colorimetric Assay Kit (WST-1 Method)

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E-BC-K001-M
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  • Inhibition And Production Of Superoxide Anionic Colorimetric Assay Kit (WST-1 Method)
  • Inhibition And Production Of Superoxide Anionic Colorimetric Assay Kit (WST-1 Method)
  • Inhibition And Production Of Superoxide Anionic Colorimetric Assay Kit (WST-1 Method)
€360.00 - €464.00

Description

Detection principle

Superoxide anion free radicals are produced through the reaction system of xanthine and xanthine oxidase. WST-1 (a water-soluble tetrazolium salt) can react with the generated superoxide anion to produce water-soluble formazan. When the tested sample contains the superoxide anion free radical inhibitor, it can inhibit the formation of formazan. When the tested sample contains the substance that produces superoxide anion free radical, it can promote the formation of formazan dye. By colorimetric analysis of WST-1 products, the units of activity of inhibition or production of superoxide anion radical in samples can be calculated.

Performance characteristics

Sample type Serum,plasma,urine,cells,cell culture supernatant,leucocyte
Detection method Colorimetric method
Assay type Activity
Assay time 35 min
Precision Average inter-assay CV: 5.8%Average intra-assay CV: 2%
Other instruments required Micropipettor, Multi-channel pipettor, Incubator, Vortex mixer, Centrifuge
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage Reagent 3: -20℃, others: 2-8℃
Valid period 6 months

Dilution of sample

Before the formal experiment, it needs to choose 2-3 samples for diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 30%~65% (the optimal inhibition ratio is the range of 40%~60%).

Inhibition ratio=[(A1-A2)-(A5-A6)]×100%/(A1-A2)

Adjust sampling volume: If inhibition ratio > 65%, need to dilute the sample or decrease the sampling volume than take the test. If inhibition ratio < 30%, need to increase the sampling volume.

 

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

4-7

Mouse serum

15-25

Rat serum

25-35

Human saliva

1

HepG2 culture supernatant

1

10% Rat brain tissue homogenate

150-200

10% Rat liver tissue homogenate

500-600

10% Mouse liver tissue homogenate

500-600

10% Mouse heart tissue homogenate

150-200

10% Epipremnum aureum tissue homogenate

20-30

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

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Additional Information

Detection method:
Colorimetric method
Detection instrument:
Microplate reader(440-460 nm,optimum wavelength: 450 nm)
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