MCDB 131 (without L-glutamine)

Background

MCDB is a low-protein and serum-free culture medium designed for specific cells. MCDB 131 is a kind of MCDB medium designed and developed by Kendler and Ham. It was originally used for culturing human microvascular endothelial cells (HMVEC). In addition to the use of MCDB 131 medium for endothelial cells, it can also be used for other cell types, such as liver cells, smooth muscle cells, and cardiomyocytes. MCDB 131 medium is not used in traditional medium because it contains trace elements, putrescine, adenine, thymine and higher concentrations of amino acids and vitamins. It is often used with EGF, hydrocortisone, glutamine and low-concentration serum together, and the added concentration of serum must be determined by cell type.