SARS-CoV-2 (COVID-19) ORF8 Antibody | 9287

SARS-CoV-2 (COVID-19) ORF8 Antibody | 9287 | ProSci

Host: Rabbit

Reactivity: Virus

Homology: N/A

Immunogen: Anti-SARS-CoV-2 (COVID-19) ORF8 antibody (9287) was raised against a peptide corresponding to 14 amino acids near carboxyl terminus of SARS-CoV-2 (COVID-19) ORF8 protein.
The immunogen is located within the last 50 amino acids of the SARS-CoV-2 (COVID-19) ORF8 protein.

Research Area: Infectious Disease, COVID-19

Tested Application: E, WB, IHC

Application: WB: 1 μg/mL; IHC: 0.1 μg/mL
Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) ORF8 antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 ORF8 recombinant protein by ELISA and WB. All other applications and species not yet tested.

Specificiy: ORF8 Antibody is predicted to not cross-react with other coronavirus family members.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 14kD

Validation: N/A

Isoform: N/A

Purification: SARS-CoV-2 (COVID-19) ORF8 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SARS-CoV-2 (COVID-19) ORF8 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SARS-CoV-2 (COVID-19) ORF8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: ORF8 protein, ns8, ORF8, Non-structural protein 8

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Coronavirus disease 2019 (COVID-19) , formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1) . The disease is the cause of the 2019–20 coronavirus outbreak (2) . SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S) , hemagglutinin-esterease dimer (HE) , a membrane glycoprotein (M) , an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16) , while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF8, ORF9b, ORF9c and ORF10. ORF8 may play a role in modulating host immune response (Probable) . May play a role in blocking host IL17 cytokine by its interaction with host IL17RA (3) .