SARS-CoV-2 (COVID-19) ORF9b Antibody

SARS-CoV-2 (COVID-19) ORF9b Antibody | 9191 | ProSci

Host: Rabbit

Reactivity: Virus

Homology: Predicted reactivity based on immunogen sequence: SARS-CoV ORF9b proteins: (50%)

Immunogen: Anti-SARS-CoV-2 (COVID-19) ORF9b antibody (9191) was raised against a peptide corresponding to 14 amino acids near the amino terminus of SARS-CoV-2 (COVID-19) ORF9b protein.
The immunogen is located in the first 50 amino acids of the SARS-CoV-2 (COVID-19) ORF9b protein.

Research Area: Infectious Disease, COVID-19

Tested Application: E, WB

Application: WB: 2 μg/mL
Antibody validated: SARS-CoV-2 (COVID-19) ORF9b antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: SARS-CoV-2 (COVID-19) ORF9b Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SARS-CoV-2 (COVID-19) ORF9b Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SARS-CoV-2 (COVID-19) ORF9b antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Accessory protein 9b, ORF-9b, Protein 9b

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Coronavirus disease 2019 (COVID-19) , formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1) . The disease is the cause of the 2019–20 coronavirus outbreak (2) . The structure of 2019-nCoV consists of the following: a spike protein (S) , hemagglutinin-esterease dimer (HE) , a membrane glycoprotein (M) , an envelope protein (E) a nucleoclapid protein (N) and RNA. ORF9b plays a role in the inhibition of host innate immune response by targeting the mitochondrial-associated adapter MAVS. Mechanistically, it usurps the E3 ligase ITCH to trigger the degradation of MAVS, TRAF3, and TRAF6. In addition, it causes mitochondrial elongation by triggering ubiquitination and proteasomal degradation of dynamin-like protein 1/DNM1L (3) .