CRB2 Antibody

CRB2 Antibody | 7155 | ProSci

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: Rabbit polyclonal CRB2 antibody was raised against a 17 amino acid peptide near the center of human CRB2.
The immunogen is located within amino acids 460 - 510 of CRB2.

Research Area: Stem Cell

Tested Application: E, WB

Application: CRB2 antibody can be used for detection of CRB2 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in rat samples. All other applications and species not yet tested.

Specificiy: At least three isoforms of CRB2 are known to exist; this antibody will detect all three. CRB2 antibody is predicted to not cross-react with other members of the CRB family.

Positive Control 1: Cat. No. 1463 - Rat Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 105, 129, and 141 kDa
Observed: 103, 128 kDa

Validation: N/A

Isoform: N/A

Purification: CRB2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: CRB2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: CRB2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: CRB2 Antibody: Protein crumbs homolog 2, Crumbs-like protein 2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: CRB2 Antibody: CRB2 (Crumbs homolog 2) , like its homologs CRB1 and CRB3, is similar to the Drosophila crumbs protein and is expressed in retina, brain and kidney. Along with other proteins, the Crumbs proteins form a complex that help set up cell polarity in developing neuroepithelial cells. At the onset of neural specification, embryonic stem cells (ESCs) upregulate CRB2, which then localizes apically in neural rosettes. Gain- and loss-of-function studies of CRB2 have shown that CRB2 is essential for the stabilization of other polarity proteins. Unlike CRB1, mutations in CRB2 do not appear to play a role in retinitis pigmentosa or in Leber congenital amaurosis.