Elabscience
Total Oxidant Status (TOS) Colorimetric Assay Kit
- SKU:
- E-BC-K802-M
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
Detection principle
Under acid conditions, the oxidizing material in the sample can oxidize Fe2+ to Fe3+, which binds highly with xylenol orange to produce a blue-purple complex. When the pH of solution is in the range of 2-3, its maximum absorption wavelength is around 590 nm, and the color depth is proportional to the content of oxidation substances in a certain concentration and a certain time, so as to indirectly calculate the total oxidation state of the sample.
Performance characteristics
| Synonyms | TOS |
| Sample type | tissue, serum and other liquid samples |
| Sensitivity | 2.5 μmol H2O2 Equiv./L |
| Detection range | 2.5-100 μmol H2O2 Equiv./L |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 30 min |
| Precision | Average inter-assay CV: 3.5%Average intra-assay CV: 2.3% |
| Other instruments required | Micropipettor, 37℃ incubator. |
| Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 3 months |
Dilution of sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (2.5-100 μmol H2O2 Equiv./L).
The recommended dilution factor for different samples is as follows (for reference only):
|
Sample type |
Dilution factor |
|
Human serum |
1 |
|
Porcine serum |
1 |
|
Horse serum |
1 |
|
Cynomolgus macaques serum |
1 |
|
10% Rat liver tissue homogenate |
1 |
|
10% Rat kidney tissue homogenate |
1 |
|
10% Rat lung tissue homogenate |
1 |
|
10% Rat spleen tissue homogenate |
1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Additional Information
Detection method: |
Colorimetric method |
Detection instrument: |
Microplate reader(580 nm-590 nm,optimum wavelength: 590 nm) |
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