CTGF(Connective tissue growth factor) Introduction

CTGF, also known as CCN2 or connective tissue growth factor, is a matricellular protein of the CCN family of extracellular matrix-associated heparin-binding proteins (see also CCN intercellular signaling protein). CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers.

Human CTGF(Connective tissue growth factor) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concent Humanion of  CTGF(Connective tissue growth factor).CTGF(Connective tissue growth factor)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilizedantibody. A sandwich complex is formed by additional  CTGF(Connective tissue growth factor) antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming optical signal measured by microplate reader.The concentHumanion of  CTGF(Connective tissue growth factor) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.  

References

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