Rat IL-1β(Interleukin 1 Beta) ELISA Kit Basic Information 

Feiyue's Rat IL-1β is an ELISA reagent for detection of IL-1β antibody, plasma or cell with sensitivity, specificity and consistency.

Rat IL-1β(Interleukin 1 Beta) Introduction

IL-1β is a cytokine protein. There are two genes for interleukin-1 (IL-1): IL-1 alpha and IL-1 beta (this gene). IL-1β precursor is cleaved by cytosolic caspase 1 (interleukin 1 beta convertase) to form mature IL-1β. The fever-producing property of Rat leukocytic pyrogen (interleukin 1) was purified by Dinarello in 1977 with a specific activity of 10–20 nanograms/kg. In 1979, Dinarello reported that purified Rat leukocytic pyrogen was the same molecule that was described by Igal Gery in 1972. He named it lymphocyte-activating factor (LAF) because it was a lymphocyte mitogen. It was not until 1984 that interleukin 1 was discovered to consist of two distinct proteins, now called interleukin-1 alpha and interleukin-1 beta.

Rat IL-1β(Interleukin 1 Beta) ELISA Kit Test Method

This kit uses sandwich ELISA to detect the concentration of Rat IL-1β.

Rat IL-1β-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Rat IL-1β antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Rat IL-1β  in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1. GRCh38: Ensembl release 89: ENSG00000125538 - Ensembl, May 2017.
2. GRCm38: Ensembl release 89: ENSMUSG00000027398 - Ensembl, May 2017.

3."Rat PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.