Feiyuebio

Mouse IL-1β(Interleukin 1 Beta) ELISA Kit

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SKU:
FY-EM3958
Weight:
0 KGS
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  • Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
  • Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
€601.12

Description

Mouse IL-1β(Interleukin 1 Beta) ELISA Kit Basic Information 

Product Name

Mouse IL-1β(Interleukin 1 Beta) ELISA Kit

Catalog NO.

FY-EM3958

Alias

IL-1β ELISA Kit, Interleukin 1 Beta ELISA Kit, IL-1beta ELISA Kit, IL1B ELISA Kit, IL1-BETA ELISA Kit, IL1F2 ELISA Kit, catabolin ELISA Kit

Application

IL-1β ELISA Kit allows for the in vitro quantitative determination of IL-1beta concentrations in serum, plasma, tissue homogenates and other biological fluids

Size

48T, 96T

Storage

2-8 ℃ for 6 months

Sensitivity

<7.5pg/ml

Species

Mouse

UniProt ID

P10749

CV(%

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

 

Feiyue's Mouse IL-1β is an ELISA reagent for detection of IL-1β antibody, plasma or cell with sensitivity, specificity and consistency.

Mouse IL-1β(Interleukin 1 Beta) Introduction

IL-1β is a cytokine protein. There are two genes for interleukin-1 (IL-1): IL-1 alpha and IL-1 beta (this gene). IL-1β precursor is cleaved by cytosolic caspase 1 (interleukin 1 beta convertase) to form mature IL-1β. The fever-producing property of Mouse leukocytic pyrogen (interleukin 1) was purified by Dinarello in 1977 with a specific activity of 10–20 nanograms/kg. In 1979, Dinarello reported that purified Mouse leukocytic pyrogen was the same molecule that was described by Igal Gery in 1972. He named it lymphocyte-activating factor (LAF) because it was a lymphocyte mitogen. It was not until 1984 that interleukin 1 was discovered to consist of two distinct proteins, now called interleukin-1 alpha and interleukin-1 beta.

Mouse IL-1β(Interleukin 1 Beta) ELISA Kit Test Method

This kit uses sandwich ELISA to detect the concentration of Mouse IL-1β.

Mouse IL-1β-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Mouse IL-1β antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Mouse IL-1β  in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

 

Reference:

  1. GRCh38: Ensembl release 89: ENSG00000125538 - Ensembl, May 2017.
  2. GRCm38: Ensembl release 89: ENSMUSG00000027398 - Ensembl, May 2017.

3."Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

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Additional Information

Size:
96T
Reactivity:
Mouse
Assay range:
12.5-800pg/ml
sensitivity:
7.5pg/ml
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