Elabscience
Reactive Oxygen Species (ROS) Fluorometric Assay Kit
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- SKU:
- E-BC-K138-F
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
Detection principle
DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.
Performance characteristics
| Synonyms | ROS, DCFH-DA, DCFH, DCF |
| Sample type | Fresh tissue,cultured cells |
| Detection method | Fluorescence method |
| Assay type | Cell-based (quantitative) |
| Assay time | 60 min |
| Other instruments required | Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge |
| Storage | Reagent 1: -20℃, Reagent 2: 2-8℃ |
| Valid period | 6 months |
Images
Increasing of ROS was observed in BPA group (B1) comparing with control group (A), and ROS of the rest of the groups plummeted to quite low values.
J Gao et al investigate the toxicity evaluation of bisphenol A (BPA) solution treatment with UV/H2O2 and UV/sodium percabonate. Reactive oxygen species (ROS) in E. coli was determined using ROS fluorometric assay kit (E-BC-K138-F).
Additional Information
Detection method: |
Fluorescence method |
Detection instrument: |
Fluorescence Microplate reader/Fluorescence Spectrophotometer /Flow Cytometry(Ex/Em=500 nm/525 nm) |
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